Recombinant human HDHD2B/LHPP protein

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, also known as LHPP, is a member of the HAD-like hydrolase superfamily. It is a unique enzyme that hydrolyzes not only oxygen-phosphorus bonds in inorganic pyrophosphate but also nitrogen-phosphorus bonds in phospholysine, phosphohistidine and imidodiphosphate in vitro. LHPP is expressed in liver, kidney and moderately in brain. Recombinant human LHPP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, HDHD2B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.5 kDa (307aa) confirmed by MALDI-TOF

Additionnal Information

LHPP, phospholysine phosphohistidine inorganic pyrophosphate phosphatase, HDHD2B, ATGP1319-10 µg, ATGP1319-20 µg, ATGP1319-50 µg, ATGP1319-100 µg, ATGP1319-250 µg, ATGP1319-500 µg, ATGP1319-1 mg, ATGP1319-10, ATGP1319-20, ATGP1319-50, ATGP1319-100, ATGP1319-250, ATGP1319-500, ATGP1319-1

References & Citations

Neff C D., et al. (2009) Mol Psychiatry. 14:621-630.; ; Chen L., et al. (2005) Front Biosci. 10:1961-1976.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl, 0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_071409.3

Uniprot Accession Number

Q9H008

Species

Human

AA Sequence

MAP WGKRLAGVRG VLLDISGVLY DSGAGGGTAI AGSVEAVARL KRSRLKVRFC TNESQKSRAE LVGQLQRLGF DISEQEVTAP APAACQILKE QGLRPYLLIH DGVRSEFDQI DTSNPNCVVI ADAGESFSYQ NMNNAFQVLM ELEKPVLISL GKGRYYKETS GLMLDVGPYM KALEYACGIK AEVVGKPSPE FFKSALQAIG VEAHQAVMIG DDIVGDVGGA QRCGMRALQV RTGKFRPSDE HHPEVKADGY VDNLAEAVDL LLQHADK