Recombinant human Importin beta/KPNB1 protein

NTF97, also known as KPNB1, is a member of the importin beta family. This protein is involved in nuclear protein import, either by associating itself with an adapter protein (for example, importin-alpha subunit which binds to nuclear localization signals (NLS) in cargo substrates), or by acting autonomously as a nuclear transport receptor (serves as NLS receptor, docking of the importin/substrate complex to the nuclear pore complex) Recombinant human KPNB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Importin subunit beta-1, IMB1, Impnb, IPO1, IPOB, NTF97

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

99.6 kDa (899aa)

Additionnal Information

Importin b, KPNB1, Importin subunit beta-1, IMB1, Impnb, IPO1, IPOB, NTF97, ATGP1316-10 µg, ATGP1316-20 µg, ATGP1316-50 µg, ATGP1316-100 µg, ATGP1316-250 µg, ATGP1316-500 µg, ATGP1316-1 mg, ATGP1316-10, ATGP1316-20, ATGP1316-50, ATGP1316-100, ATGP1316-250, ATGP1316-500, ATGP1316-1

References & Citations

Moroianu J., et al. (1995) Proc Natl Acad Sci. 92:2008-2011.; ; Moroianu J., et al. (1996) Proc Natl Acad Sci. 93:6572-6576.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002256.2

Uniprot Accession Number

Q14974

Species

Human

AA Sequence

MELITIL EKTVSPDRLE LEAAQKFLER AAVENLPTFL VELSRVLANP GNSQVARVAA GLQIKNSLTS KDPDIKAQYQ QRWLAIDANA RREVKNYVLQ TLGTETYRPS SASQCVAGIA CAEIPVNQWP ELIPQLVANV TNPNSTEHMK ESTLEAIGYI CQDIDPEQLQ DKSNEILTAI IQGMRKEEPS NNVKLAATNA LLNSLEFTKA NFDKESERHF IMQVVCEATQ CPDTRVRVAA LQNLVKIMSL YYQYMETYMG PALFAITIEA MKSDIDEVAL QGIEFWSNVC DEEMDLAIEA SEAAEQGRPP EHTSKFYAKG ALQYLVPILT QTLTKQDEND DDDDWNPCKA AGVCLMLLAT CCEDDIVPHV LPFIKEHIKN PDWRYRDAAV MAFGCILEGP EPSQLKPLVI QAMPTLIELM KDPSVVVRDT AAWTVGRICE LLPEAAINDV YLAPLLQCLI EGLSAEPRVA SNVCWAFSSL AEAAYEAADV ADDQEEPATY CLSSSFELIV QKLLETTDRP DGHQNNLRSS AYESLMEIVK NSAKDCYPAV QKTTLVIMER LQQVLQMESH IQSTSDRIQF NDLQSLLCAT LQNVLRKVQH QDALQISDVV MASLLRMFQS TAGSGGVQED ALMAVSTLVE VLGGEFLKYM EAFKPFLGIG LKNYAEYQVC LAAVGLVGDL CRALQSNIIP FCDEVMQLLL ENLGNENVHR SVKPQILSVF GDIALAIGGE FKKYLEVVLN TLQQASQAQV DKSDYDMVDY LNELRESCLE AYTGIVQGLK GDQENVHPDV MLVQPRVEFI LSFIDHIAGD EDHTDGVVAC AAGLIGDLCT AFGKDVLKLV EARPMIHELL TEGRRSKTNK AKTLATWATK ELRKLKNQA