Recombinant human p38 gamma/MAPK12 protein

Mitogen-activated protein kinase 12, also known as MAPK12, belongs to the MAP kinase family. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Recombinant human MAPK12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 12, ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

44.1 kDa (387aa) confirmed by MALDI-TOF

Additionnal Information

P38 g, MAPK12, Mitogen-activated protein kinase 12, ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3, ATGP1313-10 µg, ATGP1313-20 µg, ATGP1313-50 µg, ATGP1313-100 µg, ATGP1313-250 µg, ATGP1313-500 µg, ATGP1313-1 mg, ATGP1313-10, ATGP1313-20, ATGP1313-50, ATGP1313-100, ATGP1313-250, ATGP1313-500, ATGP1313-1

References & Citations

Han J., et al. (1995) Biochim Biophys Acta. 1265:224-227.; ; Court N W., et al. (2002) J Mol Cell Cardiol. 34:413-426.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002960

Uniprot Accession Number

P53778

Species

Human

AA Sequence

MSSPPPARSG FYRQEVTKTA WEVRAVYRDL QPVGSGAYGA VCSAVDGRTG AKVAIKKLYR PFQSELFAKR AYRELRLLKH MRHENVIGLL DVFTPDETLD DFTDFYLVMP FMGTDLGKLM KHEKLGEDRI QFLVYQMLKG LRYIHAAGII HRDLKPGNLA VNEDCELKIL DFGLARQADS EMTGYVVTRW YRAPEVILNW MRYTQTVDIW SVGCIMAEMI TGKTLFKGSD HLDQLKEIMK VTGTPPAEFV QRLQSDEAKN YMKGLPELEK KDFASILTNA SPLAVNLLEK MLVLDAEQRV TAGEALAHPY FESLHDTEDE PQVQKYDDSF DDVDRTLDEW KRVTYKEVLS FKPPRQLGAR VSKETPL