Recombinant human SHMT1 protein

Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits. Recombinant human SHMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serine hydroxymethyltransferase 1, CSHMT, SHMT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

55.2 kDa (503aa)

Additionnal Information

SHMT1, Serine hydroxymethyltransferase 1, CSHMT, SHMT, ATGP1310-10 µg, ATGP1310-20 µg, ATGP1310-50 µg, ATGP1310-100 µg, ATGP1310-250 µg, ATGP1310-500 µg, ATGP1310-1 mg, ATGP1310-10, ATGP1310-20, ATGP1310-50, ATGP1310-100, ATGP1310-250, ATGP1310-500, ATGP1310-1

References & Citations

Herbig K., et al. (2002) J Biol Chem. 277:38381-38389.; ; Trivedi V., et al. (2002) J Biol Chem. 277:17161-17169.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

AAH07979

Uniprot Accession Number

P34896

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTMPVNGAHK DADLWSSHDK MLAQPLKDSD VEVYNIIKKE SNRQRVGLEL IASENFASRA VLEALGSCLN NKYSEGYPGQ RYYGGTEFID ELETLCQKRA LQAYKLDPQC WGVNVQPYSG SPANFAVYTA LVEPHGRIMG LDLPDGGHLT HGFMTDKKKI SATSIFFESM PYKVNPDTGY INYDQLEENA RLFHPKLIIA GTSCYSRNLE YARLRKIADE NGAYLMADMA HISGLVAAGV VPSPFEHCHV VTTTTHKTLR GCRAGMIFYR KGVKSVDPKT GKEILYNLES LINSAVFPGL QGGPHNHAIA GVAVALKQAM TLEFKVYQHQ VVANCRALSE ALTELGYKIV TGGSDNHLIL VDLRSKGTDG GRAEKVLEAC SIACNKNTCP GDRSALRPSG LRLGTPALTS RGLLEKDFQK VAHFIHRGIE LTLQIQSDTG VRATLKEFKE RLAGDKYQAA VQALREEVES FASFFPLPGL PDF