Recombinant human PSMA6 protein
Proteasome subunit alpha type 6, also known as PSMA6, is a member of the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. PSMA6 is distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome subunit alpha type 6, IOTA, p27K, PROS27
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.9 kDa (270aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nandi D., et al. (2006) J Biosci. 31:137-155.; ; Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_002782
Uniprot Accession Number
P60900
Species
Human
AA Sequence
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