Recombinant human PSMG4 protein

Proteasome assembly chaperone 4, also known as PSMG4, is a chaperone protein which promotes assembly of the 20S proteasome. It interacts with PSMG3, as well as directly alpha and beta subunits of the 20S proteasome but dissociates before the formation of half-proteasomes, probably upon recruitment of POMP. Recombinant human PSMG4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome assembly chaperone 4, PAC4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

15.9 kDa (143aa) confirmed by MALDI-TOF

Additionnal Information

PSMG4, Proteasome assembly chaperone 4, PAC4, ATGP1307-10 µg, ATGP1307-20 µg, ATGP1307-50 µg, ATGP1307-100 µg, ATGP1307-250 µg, ATGP1307-500 µg, ATGP1307-1 mg, ATGP1307-10, ATGP1307-20, ATGP1307-50, ATGP1307-100, ATGP1307-250, ATGP1307-500, ATGP1307-1

References & Citations

Le Tallec B., et al. (1998) Mol Cell. 17 (4) :660-74.; ; Ramos PC., et al. (1998) Cell. 20 (4) : 489-99.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001122063

Uniprot Accession Number

Q5JS54

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEGLVVAAGG DVSLHNFSAR LWEQLVHFHV MRLTDSLFLW VGATPHLRNL AVAMCSRYDS IPVSTSLLGD TSDTTSTGLA QRLARKTNKQ VFVSYNLQNT DSNFALLVEN RIKEEMEAFP EKF