Recombinant human PA28 gamma/PSME3 protein

PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome activator subunit 3, 11S regulator complex subunit gamma, REG-gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, PA28g, PA28gamma, Ki

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.7 kDa (274aa) confirmed by MALDI-TOF

Additionnal Information

PA28 Activator g Subunit, Proteasome activator complex subunit 3 isoform 1, Ki, PA28-gamma, PA28G, REG-GAMMA, ATGP1302-10 µg, ATGP1302-20 µg, ATGP1302-50 µg, ATGP1302-100 µg, ATGP1302-250 µg, ATGP1302-500 µg, ATGP1302-1 mg, ATGP1302-10, ATGP1302-20, ATGP1302-50, ATGP1302-100, ATGP1302-250, ATGP1302-500, ATGP1302-1

References & Citations

Wilk S., et al. (2000) Arch. Biochem. Biophys. 383:265-271; ; Zhang Z., et al. (2008) EMBO J. 27:852-864

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl

Scientific Category

Ubiquitination

NCBI Accession Number

NP_005780.2

Uniprot Accession Number

P61289

Species

Human

AA Sequence

MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY