Recombinant human Sulfatase Modifying Factor 1/SUMF1 protein

SuMF1 (Sulfatase-modifying factor 1) belongs to the SuMF family. SuMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant human SuMF1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.1 kDa (304aa)

Additionnal Information

UNQ3037, SUMF1, SUMF 1, Sulfatase-modifying factor1, Sulfatase-modifying factor 1, Sulfatase modifying factor1, Sulfatase modifying factor 1, Formylglycine-generating enzyme, Formylglycine generating enzyme, FGE, C-alpha-formylglycine-generating enzyme1, C-alpha-formylglycine-generating enzyme 1, C alpha formylglycine generating enzyme1, C alpha formylglycine generating enzyme 1, AAPA3037, ATGP1301-10 µg, ATGP1301-20 µg, ATGP1301-50 µg, ATGP1301-100 µg, ATGP1301-250 µg, ATGP1301-500 µg, ATGP1301-1 mg, ATGP1301-010, ATGP1301-020, ATGP1301-050, ATGP1301-100, ATGP1301-250, ATGP1301-500, ATGP1301-01M

References & Citations

Fraldi A., et al. (2007) Biochem J. 403 (2) :305-12.; ; Cosma MP., et al. (2003) Cell 113 (4) : 445-56.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol, 2mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_877437

Uniprot Accession Number

Q8NBK3

Species

Human

AA Sequence

MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD