Recombinant human p38 alpha/MAPK14 protein

MAPK14 (Mitogen-activated protein kinase 14) is a member of the MAP kinase family. MAPK14 is most closely related to p38 MAP kinases (MAPKs) . MAPKs are activated primarily in response to inflammatory cytokines and cellular stress, and inhibitors which target the MAPK14 and MAPK11 have shown potential for the treatment of inflammatory disease. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. Recombinant human MAPK14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

43.7 kDa (383aa) confirmed by MALDI-TOF

Additionnal Information

P38 a, MAPK14, Mitogen-activated protein kinase 14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A, ATGP1300-10 µg, ATGP1300-20 µg, ATGP1300-50 µg, ATGP1300-100 µg, ATGP1300-250 µg, ATGP1300-500 µg, ATGP1300-1 mg, ATGP1300-10, ATGP1300-20, ATGP1300-50, ATGP1300-100, ATGP1300-250, ATGP1300-500, ATGP1300-1

References & Citations

Beardmore VA, et al. (2005) Mol Cell Biol. 25 (23) :10454-64.; ; Chen, Z, et al (2001) J. Biol. Chem. (united States) . 276 (19) : 16070-5

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_620581.1

Uniprot Accession Number

Q16539

Species

Human

AA Sequence

MSQERPT FYRQELNKTI WEVPERYQNL SPVGSGAYGS VCAAFDTKTG LRVAVKKLSR PFQSIIHAKR TYRELRLLKH MKHENVIGLL DVFTPARSLE EFNDVYLVTH LMGADLNNIV KCQKLTDDHV QFLIYQILRG LKYIHSADII HRDLKPSNLA VNEDCELKIL DFGLARHTDD EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL TGRTLFPGTD HIDQLKLILR LVGTPGAELL KKISSESARN YIQSLTQMPK MNFANVFIGA NPLAVDLLEK MLVLDSDKRI TAAQALAHAY FAQYHDPDDE PVADPYDQSF ESRDLLIDEW KSLTYDEVIS FVPPPLDQEE MES