Recombinant human p38 beta/MAPK11 protein

MAPK11 (Mitogen-activated protein kinase 11) is a member of the MAP kinase family. This kinase is most closely related to p38 MAP kinases (MAPKs) . MAPKs are activated primarily in response to inflammatory cytokines and cellular stress, and inhibitors which target the MAPK14 and MAPK11 have shown potential for the treatment of inflammatory disease. MAPK11 has been shown to interact with HDAC3 and Promyelocytic leukemia protein. This kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Recombinant human MAPK11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 11, p38-2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

43.7 kDa (387aa) confirmed by MALDI-TOF

Additionnal Information

P38 b, MAPK11, Mitogen-activated protein kinase 11, p38-2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B, ATGP1299-10 µg, ATGP1299-20 µg, ATGP1299-50 µg, ATGP1299-100 µg, ATGP1299-250 µg, ATGP1299-500 µg, ATGP1299-1 mg, ATGP1299-10, ATGP1299-20, ATGP1299-50, ATGP1299-100, ATGP1299-250, ATGP1299-500, ATGP1299-1

References & Citations

Beardmore VA, et al. (2005) Mol Cell Biol. 25 (23) :10454-64.; ; Mahlknecht u, et al. (2004) J Immunol. 173 (6) :3979-90.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002742.3

Uniprot Accession Number

Q15759

Species

Human

AA Sequence

MSGPRAG FYRQELNKTV WEVPQRLQGL RPVGSGAYGS VCSAYDARLR QKVAVKKLSR PFQSLIHARR TYRELRLLKH LKHENVIGLL DVFTPATSIE DFSEVYLVTT LMGADLNNIV KCQALSDEHV QFLVYQLLRG LKYIHSAGII HRDLKPSNVA VNEDCELRIL DFGLARQADE EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL QGKALFPGSD YIDQLKRIME VVGTPSPEVL AKISSEHART YIQSLPPMPQ KDLSSIFRGA NPLAIDLLGR MLVLDSDQRV SAAEALAHAY FSQYHDPEDE PEAEPYDESV EAKERTLEEW KELTYQEVLS FKPPEPPKPP GSLEIEQ