Recombinant human TUBG1 protein

TuBG1 is a member of the tubulin superfamily. This protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. It mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. Recombinant human TuBG1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Tubulin gamma-1 chain, GCP-1, TuBG, TuBGCP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

53.3 kDa (471aa)

Additionnal Information

TUBG1, Tubulin gamma-1 chain, GCP-1, TuBG, TuBGCP1, ATGP1295-10 µg, ATGP1295-20 µg, ATGP1295-50 µg, ATGP1295-100 µg, ATGP1295-250 µg, ATGP1295-500 µg, ATGP1295-1 mg, ATGP1295-10, ATGP1295-20, ATGP1295-50, ATGP1295-100, ATGP1295-250, ATGP1295-500, ATGP1295-1

References & Citations

Zheng Y., et al. (1991) Cell. 65 (5) :817-23.; ; Wise DO., et al. (2000) Genomics. 67 (2) :164-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 1M urea, 5% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_001061

Uniprot Accession Number

P23258

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE Q