Recombinant human TAX1BP3 protein

TAX1BP3, also known as Tax1-binding protein 3, contains one PDZ domain, through which most of its interactions with other proteins are made. The PDZ domain of TAX1BP3 interacts with the C-terminus of Rhotekin to facilitate Rho-mediated activation of the FOS serum response element. The PDZ domain also interacts with beta-catenin to inhibit the transcriptional activity of beta-catenin. It has been detected in a variety of cell lines, including HeLa, and is weakly expressed in peripheral blood leukocytes. Recombinant human TAX1BP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Tax1-binding protein 3, TIP-1, TIP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

15.8 kDa (144aa) confirmed by MALDI-TOF

Additionnal Information

TAX1BP3, Tax1-binding protein 3, TIP-1, TIP1, ATGP1294-10 µg, ATGP1294-20 µg, ATGP1294-50 µg, ATGP1294-100 µg, ATGP1294-250 µg, ATGP1294-500 µg, ATGP1294-1 mg, ATGP1294-10, ATGP1294-20, ATGP1294-50, ATGP1294-100, ATGP1294-250, ATGP1294-500, ATGP1294-1

References & Citations

Rousset R. et al. (1998) Oncogene.16: 643-654; ; Reynaud C. et al. (2000) J. Biol. Chem. 275: 33962-33968.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055419

Uniprot Accession Number

O14907

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS