Recombinant human STAR protein

STAR, also known as steroidogenic acute regulatory protein, appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. It increases pregnenolone synthesis more than four-fold and a major STAR transcript of 1. 6 kb is found in ovary and testis. During ongoing growth and differentiation of the follicle of the ovary, the immunoreactivity of it tends to shift from the granulosa cells of early antral follicles to the theca cell layers in the adult. Recombinant human STAR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Steroidogenic acute regulatory protein mitochondrial, Steroidogenic acute regulatory protein, mitochondrial

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.1 kDa (243aa) confirmed by MALDI-TOF

Additionnal Information

STAR, Steroidogenic acute regulatory protein mitochondrial, Steroidogenic acute regulatory protein, mitochondrial, ATGP1292-10 µg, ATGP1292-20 µg, ATGP1292-50 µg, ATGP1292-100 µg, ATGP1292-250 µg, ATGP1292-500 µg, ATGP1292-1 mg, ATGP1292-10, ATGP1292-20, ATGP1292-50, ATGP1292-100, ATGP1292-250, ATGP1292-500, ATGP1292-1

References & Citations

Borthwick F. et al. (2009) FEBS Lett. 583:1147-1153.; ; Mikhaylova I.V. et al. (2008) J. Endocrinol.199: 435-444.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_000340.2

Uniprot Accession Number

P49675

Species

Human

AA Sequence

EETLYSDQE LAYLQQGEEA MQKALGILSN QEGWKKESQQ DNGDKVMSKV VPDVGKVFRL EVVVDQPMER LYEELVERME AMGEWNPNVK EIKVLQKIGK DTFITHELAA EAAGNLVGPR DFVSVRCAKR RGSTCVLAGM ATDFGNMPEQ KGVIRAEHGP TCMVLHPLAG SPSKTKLTWL LSIDLKGWLP KSIINQVLSQ TQVDFANHLR KRLESHPASE ARC