Recombinant human PA28 beta/PSME2 protein

PSME2, also known as proteasome (prosome, macropain) activator subunit 2, is an IFN-gamma inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. Downregulation of PSME2 results in abnormal proteasome activation and has been implicated in the development of intimal hyperplasia (IH) in animal models. Recombinant human PSME2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Proteasome activator subunit 2, 11S regulator complex subunit beta, REG-beta, Activator of multicatalytic protease subunit 2, Proteasome activator 28 subunit beta, PA28b, PA28beta

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.5 kDa (259aa) confirmed by MALDI-TOF

Additionnal Information

PA28 Activator b Subunit, Proteasome (prosome macropain) activator subunit 2, Proteasome (prosome, macropain) activator subunit 2, PA28B, PA28beta, REGbeta, ATGP1288-10 µg, ATGP1288-20 µg, ATGP1288-50 µg, ATGP1288-100 µg, ATGP1288-250 µg, ATGP1288-500 µg, ATGP1288-1 mg, ATGP1288-10, ATGP1288-20, ATGP1288-50, ATGP1288-100, ATGP1288-250, ATGP1288-500, ATGP1288-1

References & Citations

Kohda K. et al. (1998) J. Immunol. 160: 4923-4935.; ; Fabunmi R.P. et al. (2001) J. Cell. Sci. 114: 29-36

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Ubiquitination

NCBI Accession Number

AAH72025.1

Uniprot Accession Number

Q9UL46

Species

Human

AA Sequence

MAKPCGVRLS GEARKQVEVF RQNLFQEAEE FLYRFLPQKI IYLNQLLQED SLNVADLTSL RAPLDIPIPD PPPKDDEMET DKQEKKEVPK CGFLPGNEKV LSLLALVKPE VWTLKEKCIL VITWIQHLIP KIEDGNDFGV AIQEKVLERV NAVKTKVEAF QTTISKYFSE RGDAVAKASK ETHVMDYRAL VHERDEAAYG ELRAMVLDLR AFYAELYHII SSNLEKIVNP KGEEKPSMY