Recombinant human PSMA3 protein
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. Recombinant human PSMA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome (prosome macropain) subunit alpha type 3, Proteasome (prosome, macropain) subunit, alpha type 3, HC8, MGC12306, MGC32631, PSC3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.5 kDa (275aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kristensen P., et al. (1995) Biochem Biophys Res Commun. 207 (3) :1059.; ; Wenzel T., et al. (1995) Nat Struct Biol. 2 (3) :199-204.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
Scientific Category
Ubiquitination
NCBI Accession Number
NP_002779
Uniprot Accession Number
P25788
Species
Human
AA Sequence
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