Recombinant human NACA protein

NACA is member of the nascent polypeptide associated complex (NAC) alpha subunit family that participate in preventing inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER) . Most NACA proteins localize to both nucleus as well as cytoplasm, and contain NAC-A/B (NAC-alpha/beta) and uBA (ubiquitin-associated) domains. The uBA domain is associated with proteins involved in the ubiquitin-proteasome pathway for protein degradation. Recombinant human NACA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nascent polypeptide-associated complex alpha subunit, HSD48, MGC117224, NACA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.5 kDa (235aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

NACA, Nascent polypeptide-associated complex alpha subunit, HSD48, MGC117224, NACA1, ATGP1286-10 µg, ATGP1286-20 µg, ATGP1286-50 µg, ATGP1286-100 µg, ATGP1286-250 µg, ATGP1286-500 µg, ATGP1286-1 mg, ATGP1286-10, ATGP1286-20, ATGP1286-50, ATGP1286-100, ATGP1286-250, ATGP1286-500, ATGP1286-1

References & Citations

Lopez S., et al. (2005) J Cell Sci. 118 (8) :1595-605.; ; Stilo R., et al. (2003) Biochem Biophys Res Commun. 303 (4) :1034-41.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001106672.1

Uniprot Accession Number

Q13765

Species

Human

AA Sequence

MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM