Recombinant human GMDS protein

GMDS, also known as SDR3E1, belongs to the GDP-mannose 4, 6-dehydratase family. This protein utilizes NADP as a cofactor to catalyze the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose. Mutations in the gene encoding GMDS are involved in resistance to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) -induced apoptosis. Recombinant human GMDS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

GDP-mannose 46 dehydratase, GDP-mannose 4,6 dehydratase, GMD, SDR3E1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

44.1 kDa (392aa) confirmed by MALDI-TOF

Additionnal Information

GMDS, ATGP1282-10 µg, ATGP1282-20 µg, ATGP1282-50 µg, ATGP1282-100 µg, ATGP1282-250 µg, ATGP1282-500 µg, ATGP1282-1 mg, ATGP1282-10, ATGP1282-20, ATGP1282-50, ATGP1282-100, ATGP1282-250, ATGP1282-500, ATGP1282-1

References & Citations

Ohyama C. et al. (1998) J Biol Chem. 273: 14582-14587.; ; Moriwaki K. et al. (2009) Gastroenterology 137: 188-198.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

NP_001491

Uniprot Accession Number

O60547

Species

Human

AA Sequence

MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH ADVELMRTNP NA