Recombinant human IDI1 protein

Isopentenyl-diphosphate isomerase 1, also known as IDI1, is a member of the IPP isomerase type I family and is involved in cholesterol biosynthesis. IDI1 is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which is the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. Recombinant human IDI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Isopentenyl-diphosphate isomerase 1, IPP1, IPPI1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.6 kDa (248aa) confirmed by MALDI-TOF

Additionnal Information

IDI1, Isopentenyl-diphosphate isomerase 1, IPP1, IPPI1, ATGP1277-10 µg, ATGP1277-20 µg, ATGP1277-50 µg, ATGP1277-100 µg, ATGP1277-250 µg, ATGP1277-500 µg, ATGP1277-1 mg, ATGP1277-10, ATGP1277-20, ATGP1277-50, ATGP1277-100, ATGP1277-250, ATGP1277-500, ATGP1277-1

References & Citations

Hahn F M., et al. (1996) Arch Biochem Biophys. 332:30-34.; ; Zheng W., et al. (2007) J Mol Biol. 366:1447-1458.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. PBS containing 20% glycerol, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

AAH57827

Uniprot Accession Number

Q13907

Species

Human

AA Sequence

MMPEINTNHL DKQQVQLLAE MCILIDENDN KIGAETKKNC HLNENIEKGL LHRAFSVFLF NTENKLLLQQ RSDAKITFPG CFTNTCCSHP LSNPAELEES DALGVRRAAQ RRLKAELGIP LEEVPPEEIN YLTRIHYKAQ SDGIWGEHEI DYILLVRKNV TLNPDPNEIK SYCYVSKEEL KELLKKAASG EIKITPWFKI IAATFLFKWW DNLNHLNQFV DHEKIYRM