Recombinant human IDI1 protein
Isopentenyl-diphosphate isomerase 1, also known as IDI1, is a member of the IPP isomerase type I family and is involved in cholesterol biosynthesis. IDI1 is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which is the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. Recombinant human IDI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Isopentenyl-diphosphate isomerase 1, IPP1, IPPI1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
28.6 kDa (248aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hahn F M., et al. (1996) Arch Biochem Biophys. 332:30-34.; ; Zheng W., et al. (2007) J Mol Biol. 366:1447-1458.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. PBS containing 20% glycerol, 0.1mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
AAH57827
Uniprot Accession Number
Q13907
Species
Human
AA Sequence
Frequently Asked Questions
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