Recombinant human IMP Dehydrogenase 2/IMPDH2 protein
IMPDH2 belongs to the IMPDH/GMPR family. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. Also IMPDH1 and IMPDH2 are targets for the important immunosuppressive drug, mycophenolic acid (MPA) . Recombinant human IMPDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Inosine-5'-monophosphate dehydrogenase 2, IMPD2, IMPDH-II
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
58kDa (534aa)
Additionnal Information
References & Citations
Wu TY, Peng Y, et al. (2010) Pharmacol. 161 (7) :1584-98. ; ; Bremer S, et al. (2008) Transplantation. 85 (1) :55-61.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_000875
Uniprot Accession Number
P12268
Species
Human
AA Sequence
Frequently Asked Questions
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