Recombinant human Glutathione Peroxidase 1/GPX1 (U49C) protein

GPX1 (Glutathione peroxidase 1) belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. The GPX1 is part of the enzymatic antioxidant defence, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides that may contribute to prostate cancer development. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX1 (u49C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutathione peroxidase 1, GSHPX1, Selenoprotein GPX1, GPx-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.2 kDa (223aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione Peroxidase1, GPX1 (U49C), ATGP1273-10 µg, ATGP1273-20 µg, ATGP1273-50 µg, ATGP1273-100 µg, ATGP1273-250 µg, ATGP1273-500 µg, ATGP1273-1 mg, ATGP1273-10, ATGP1273-20, ATGP1273-50, ATGP1273-100, ATGP1273-250, ATGP1273-500, ATGP1273-1

References & Citations

Ichimura Y., et al. (2004) J urol.172 (2) :728-32.; ; Li S, Yan T, et al. (2000) Cancer Res.Jul 60 (14) :3927-39.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 100 mM NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000572.2

Uniprot Accession Number

P07203

Species

Human

AA Sequence

MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA