Recombinant human eIF-3 alpha/EIF3J protein

EIF3J (Eukaryotic translation initiation factor 3 subunit J) belongs to the EIF-3 subunit J family. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA entry channel of the 40S subunit, placing EIF3J directly in the ribosomal decoding center. EIF3J also interacts with eIF1A and reduces 40S subunit affinity for mRNA. A high affinity for mRNA is restored upon recruitment of initiator tRNA, even though EIF3J remains in the mRNA-binding cleft in the presence of tRNA. Recombinant human EIF3J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 3 subunit J, eIF3-alpha, eIF3-p35, EIF3S1, Eukaryotic translation initiation factor 3 subunit 1 alpha

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24 kDa (210aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Eukaryotic translation initiation factor 3, Subunit J, subunit 1 alpha, Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit 1 alpha, EIF3S1, eIF3-p35, EIF3J, eIF3-alpha, ATGP1272-10 µg, ATGP1272-20 µg, ATGP1272-50 µg, ATGP1272-100 µg, ATGP1272-250 µg, ATGP1272-500 µg, ATGP1272-1 mg, ATGP1272-010, ATGP1272-020, ATGP1272-050, ATGP1272-100, ATGP1272-250, ATGP1272-500, ATGP1272-01M

References & Citations

Fraser CS, et al. (2007) Mol Cell. 26 (6) :811-9.; ; Block K.L., et al. (1998) J. Biol. Chem. 273:31901-31908

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003749

Uniprot Accession Number

O75822

Species

Human

AA Sequence

KISEKKKIA EKIKEKERQQ KKRQEEIKKR LEEPEEPKVL TPEEQLADKL RLKKLQEESD LELAKETFGV NNAVYGIDAM NPSSRDDFTE FGKLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DLADYGGYDG GYVQDYEDFM