Recombinant human eIF-3 alpha/EIF3J protein
EIF3J (Eukaryotic translation initiation factor 3 subunit J) belongs to the EIF-3 subunit J family. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA entry channel of the 40S subunit, placing EIF3J directly in the ribosomal decoding center. EIF3J also interacts with eIF1A and reduces 40S subunit affinity for mRNA. A high affinity for mRNA is restored upon recruitment of initiator tRNA, even though EIF3J remains in the mRNA-binding cleft in the presence of tRNA. Recombinant human EIF3J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 3 subunit J, eIF3-alpha, eIF3-p35, EIF3S1, Eukaryotic translation initiation factor 3 subunit 1 alpha
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
24 kDa (210aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Fraser CS, et al. (2007) Mol Cell. 26 (6) :811-9.; ; Block K.L., et al. (1998) J. Biol. Chem. 273:31901-31908
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_003749
Uniprot Accession Number
O75822
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
