Recombinant human SYF2 protein

SYF2, also known as pre-mRNA-splicing factor SYF2, may be involved in pre-mRNA splicing. This protein interacts with cyclin D-type binding-protein 1, which is thought to be a cell cycle regulator at the G1/S transition. It is abundantly expressed in the heart, skeletal muscle and kidney and is expressed at lower levels in other tissues. Recombinant human SYF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Pre-mRNA-splicing factor SYF2, CBPIN, GCIPIP, p29

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.5 kDa (173aa) confirmed by MALDI-TOF

Additionnal Information

SYF2, Pre-mRNA-splicing factor SYF2, CBPIN, GCIPIP, p29, ATGP1269-10 µg, ATGP1269-20 µg, ATGP1269-50 µg, ATGP1269-100 µg, ATGP1269-250 µg, ATGP1269-500 µg, ATGP1269-1 mg, ATGP1269-10, ATGP1269-20, ATGP1269-50, ATGP1269-100, ATGP1269-250, ATGP1269-500, ATGP1269-1

References & Citations

Chang M.-S., et al. (2000) Biochem. Biophys. Res. Commun. 279:732-737; ; Jurica M.S., et al. (2002) RNA. 8:426-439

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA

Scientific Category

Cell Cycle

NCBI Accession Number

NP_056299

Uniprot Accession Number

O95926

Species

Human

AA Sequence

DYEKVKL LEISAEDAER WERKKKRKNP DLGFSDYAAA QLRQYHRLTK QIKPDMETYE RLREKHGEEF FPTSNSLLHG THVPSTEEID RMVIDLEKQI EKRDKYSRRR PYNDDADIDY INERNAKFNK KAERFYGKYT AEIKQNLERG TAV