Recombinant human HDDC3 protein
HDDC3, also known as guanosine-3', 5'-bis (diphosphate) -pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn (2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3', 5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Guanosine-3'5'-bis (diphosphate) -pyrophosphohydrolase MESH1, Guanosine-3',5'-bis (diphosphate) -pyrophosphohydrolase MESH1, MESH1, ppGpp
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.9 kDa (160aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sun D., et al. (2010) Nat. Struct. Mol. Biol. 17:1188-1194; ; Zody M.C., et al. (2006) Nature. 440:671-675
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_940929
Uniprot Accession Number
Q8N4P3
Species
Human
AA Sequence
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