Recombinant human DNAJB8 protein

DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de) acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation. Recombinant human DNAJB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

DnaJ homolog subfamily B member 8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.8 kDa (252aa) confirmed by MALDI-TOF

Additionnal Information

DNAJB8, DnaJ homolog subfamily B member 8, ATGP1265-10 µg, ATGP1265-20 µg, ATGP1265-50 µg, ATGP1265-100 µg, ATGP1265-250 µg, ATGP1265-500 µg, ATGP1265-1 mg, ATGP1265-10, ATGP1265-20, ATGP1265-50, ATGP1265-100, ATGP1265-250, ATGP1265-500, ATGP1265-1

References & Citations

Hageman J., et al. (2010) Mol Cell. 37 (3) :355-69.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_699161

Uniprot Accession Number

Q8NHS0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK