Recombinant human p27/Kip1 protein

CDKN1B, also known as cyclin-dependent kinase inhibitor 1B, belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. This protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle. Recombinant human CDKN1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase inhibitor 1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.2 kDa (218aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

P27, Kip1, Cyclin-dependent kinase inhibitor 1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, ATGP1264-10 µg, ATGP1264-20 µg, ATGP1264-50 µg, ATGP1264-100 µg, ATGP1264-250 µg, ATGP1264-500 µg, ATGP1264-1 mg, ATGP1264-10, ATGP1264-20, ATGP1264-50, ATGP1264-100, ATGP1264-250, ATGP1264-500, ATGP1264-1

References & Citations

Ishida N., et al. (2000) J. Biol. Chem. 275:25146-25154; ; Viglietto G., et al. (2002) Nat. Med. 8:1136-1144

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004055.1

Uniprot Accession Number

P46527

Species

Human

AA Sequence

MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK VPAQESQDVS GSRPAAPLIG APANSEDTHL VDPKTDPSDS QTGLAEQCAG IRKRPATDDS STQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT