Recombinant human AK5 protein

AK5, also known as adenylate kinase isoenzyme 5, is a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the uMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Recombinant human AK5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Adenylate kinase isoenzyme 5, AK6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

65.9 kDa (586aa)

Additionnal Information

AK5, Adenylate kinase isoenzyme 5, AK6, ATGP1263-10 µg, ATGP1263-20 µg, ATGP1263-50 µg, ATGP1263-100 µg, ATGP1263-250 µg, ATGP1263-500 µg, ATGP1263-1 mg, ATGP1263-10, ATGP1263-20, ATGP1263-50, ATGP1263-100, ATGP1263-250, ATGP1263-500, ATGP1263-1

References & Citations

Van Rompay A.R., et al. (1999) Eur. J. Biochem. 261:509-517; ; Solaroli N., et al. (2009) FEBS Lett. 583 (17) :2872-6

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

AAH36666

Uniprot Accession Number

Q9Y6K8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMNTNDA KEYMARREIP QLFESLLNGL MCSKPEDPVE YLESCLQKVK ELGGCDKVKW DTFVSQEKKT LPPLNGGQSR RSFLRNVMPG NSNFPYRRYD RLPPIHQFSI ESDTDLSETA ELIEEYEVFD PTRPRPKIIL VIGGPGSGKG TQSLKIAERY GFQYISVGEL LRKKIHSTSS NRKWSLIAKI ITTGELAPQE TTITEIKQKL MQIPDEEGIV IDGFPRDVAQ ALSFEDQICT PDLVVFLACA NQRLKERLLK RAEQQGRPDD NVKATQRRLM NFKQNAAPLV KYFQEKGLIM TFDADRDEDE VFYDISMAVD NKLFPNKEAA AGSSDLDPSM ILDTGEIIDT GSDYEDQGDD QLNVFGEDTM GGFMEDLRKC KIIFIIGGPG SGKGTQCEKL VEKYGFTHLS TGELLREELA SESERSKLIR DIMERGDLVP SGIVLELLKE AMVASLGDTR GFLIDGYPRE VKQGEEFGRR IGDPQLVICM DCSADTMTNR LLQRSRSSLP VDDTTKTIAK RLEAYYRASI PVIAYYETKT QLHKINAEGT PEDVFLQLCT AIDSIF