Recombinant human IkB-alpha protein

NF-kappa-B inhibitor alpha, also known as NFKBIA, is members of the IkB family of proteins can be divided into four groups (IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon) . NFKBIA inhibits the NFkB complex by binding and sequestering it in the cytoplasm. upon stimulation, NFKBIA is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Recombinant human NFKBIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NF-kappa-B inhibitor alpha, IKBA, MAD-3, NFKBI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.7 kDa (337aa) confirmed by MALDI-TOF

Additionnal Information

IkB-a, NF-kappa-B inhibitor alpha, IKBA, MAD-3, NFKBI, ATGP1261-10 µg, ATGP1261-20 µg, ATGP1261-50 µg, ATGP1261-100 µg, ATGP1261-250 µg, ATGP1261-500 µg, ATGP1261-1 mg, ATGP1261-10, ATGP1261-20, ATGP1261-50, ATGP1261-100, ATGP1261-250, ATGP1261-500, ATGP1261-1

References & Citations

Simson C S., et al. (2000) J Biol Chem. 275:16879-16884.; ; Singh S., et al. (1996) J Biol Chem. 271:31049-31054.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_065390.1

Uniprot Accession Number

P25963

Species

Human

AA Sequence

MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL