Recombinant human ARFRP2/ARL15 protein

ARL15, also known as ARFRP2, belongs to the ARF family. ARFs are important in eukaryotic vesicular trafficking pathways and play an essential role in the activation of phospholipase D. ARL15 variants influence levels of Acrp30, an adipocyte-derived protein that is particularly heritable and inversely associated with risk of type 2 diabetes mellitus and coronary heart disease. Recombinant human ARL15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADP-ribosylation factor-like protein 15, ARFRP2, ADP ribosylation factor like GTPase 15, ADP-ribosylation factor related protein 2, ARF-related protein 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25 kDa (224aa) confirmed by MALDI-TOF

Additionnal Information

ARL15, ADP-ribosylation factor-like protein 15, ARFRP2, ATGP1257-10 µg, ATGP1257-20 µg, ATGP1257-50 µg, ATGP1257-100 µg, ATGP1257-250 µg, ATGP1257-500 µg, ATGP1257-1 mg, ATGP1257-10, ATGP1257-20, ATGP1257-50, ATGP1257-100, ATGP1257-250, ATGP1257-500, ATGP1257-1

References & Citations

Richards J B., et al. (2009) PLoS Genet. 5:e1000768.; ; Gillingham A K., et al. (2007) Annu Rev Cell Dev Biol. 23:579-611.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_061960

Uniprot Accession Number

Q9NXU5

Species

Human

AA Sequence

MSDLRITEAF LYMDYLCFRA LCCKGPPPAR PEYDLVCIGL TGSGKTSLLS KLCSESPDNV VSTTGFSIKA VPFQNAILNV KELGGADNIR KYWSRYYQGS QGVIFVLDSA SSEDDLEAAR NELHSALQHP QLCTLPFLIL ANHQDKPAAR SVQEIKKYFE LEPLARGKRW ILQPCSLDDM DALKDSFSQL INLLEEKDHE AVRM