Recombinant human DNA polymerase lambda/POLL protein
DNA polymerase lambda, also known as POLL, is a DNA polymerase involved in base excision repair (BER) . POLL is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. In addition, POLL may play a role in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Recombinant human POLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA polymerase lambda, BETA-N, POL-KAPPA, Pol beta2, POLKAPPA
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36 kDa (320aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
O Wang J., et al. (2001) Cancer Res. 61:5366-5369.; ; Zhang Y., et al. (2000) Nucleic Acids Res. 28:4147-4156.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
AAH03548
Uniprot Accession Number
Q9UGP5
Species
Human
AA Sequence
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