Recombinant human RPS3 protein
40S ribosomal protein S3, also known as RPS3, belongs to the S3P family of ribosomal proteins. RPS3 mediates the formation of the mRNA binding site 3' of the codon in the decoding site. In addition, RPS3 is involved in DNA damage recognition as shown by its affinity for abasic sites and 7, 8-dihydro-8-oxoguanine residues and its interaction with human base excision repair proteins OGG1 and Ref-1. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. Recombinant human RPS3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
40S ribosomal protein S3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
28.8 kDa (263aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Hegde V (2006) DNA Repair. 5:810-815.; ; Choi S H., et al. (2006) J Biochem Mol Biol. 39:208-215.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000996.2
Uniprot Accession Number
P23396
Species
Human
AA Sequence
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