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Recombinant human Betacellulin protein

BTC (Betacellulin) is a member of the EGF family of growth factors and contains 1 EGF-like domain. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. The effects of BTC are probably mediated by the EGF receptor and other related receptors. Recombinant human BTC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Betacellulin, probetacellulin precursor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

11.3kD (101aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Bcellulin, Betacellulin, probetacellulin precursor, ATGP1250-10 µg, ATGP1250-20 µg, ATGP1250-50 µg, ATGP1250-100 µg, ATGP1250-250 µg, ATGP1250-500 µg, ATGP1250-1 mg, ATGP1250-10, ATGP1250-20, ATGP1250-50, ATGP1250-100, ATGP1250-250, ATGP1250-500, ATGP1250-1

References & Citations

Gratao AA, et al. (2008) Biol Reprod.78 (1) :43-52.; ; Genetos DC, et al. (2010) Cell Tissue Res. 340 (1) :81-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 200 mM NaCl

Scientific Category

Growth factors

NCBI Accession Number

NP_001720.1

Uniprot Accession Number

P35070

Species

Human

AA Sequence

DGNSTRSPE TNGLLCGDPE ENCAATTTQS KRKGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYI GARCERVDLF Y

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