Recombinant human VEGF 165 protein

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Vascular endothelial growth factor A isoform d, VPF, VEGF, VEGF-A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.4 kDa (186aa) conformed by MALDI-TOF

Additionnal Information

VEGF165, ATGP1248-10 µg, ATGP1248-20 µg, ATGP1248-50 µg, ATGP1248-100 µg, ATGP1248-250 µg, ATGP1248-500 µg, ATGP1248-1 mg, ATGP1248-10, ATGP1248-20, ATGP1248-50, ATGP1248-100, ATGP1248-250, ATGP1248-500, ATGP1248-1

References & Citations

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1) :16-24. ; ; Neufeld G., et al. (1999) FASEB J. 13 (1) :9-22.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) 5mM DTT, 50 % glycerol, 200 mM NaCl, 2mM EDTA

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001020539.2

Uniprot Accession Number

P15692

Species

Human

AA Sequence

APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR