Recombinant human VEGF 165 protein
Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Vascular endothelial growth factor A isoform d, VPF, VEGF, VEGF-A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
21.4 kDa (186aa) conformed by MALDI-TOF
Additionnal Information
References & Citations
Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1) :16-24. ; ; Neufeld G., et al. (1999) FASEB J. 13 (1) :9-22.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) 5mM DTT, 50 % glycerol, 200 mM NaCl, 2mM EDTA
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001020539.2
Uniprot Accession Number
P15692
Species
Human
AA Sequence
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