Recombinant human Serglycin/SRGN protein

SRGN is a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. Recombinant human SRGN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serglycin precursor, FLJ12930, MGC9289, PPG, PRG, PRG1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.4 kDa (156aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

SRGN, Serglycin precursor, FLJ12930, MGC9289, PPG, PRG, PRG1, ATGP1242-10 µg, ATGP1242-20 µg, ATGP1242-50 µg, ATGP1242-100 µg, ATGP1242-250 µg, ATGP1242-500 µg, ATGP1242-1 mg, ATGP1242-10, ATGP1242-20, ATGP1242-50, ATGP1242-100, ATGP1242-250, ATGP1242-500, ATGP1242-1

References & Citations

Niemann Cu., et al. (2004) J Leukoc Biol. 76 (2) :406-15.; ; Raja SM., et al. (2000) J Biol Chem. 277 (51) :49523-30.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_002718

Uniprot Accession Number

P10124

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML