Recombinant human PSMA1 protein
PSMA1 is a prosomal protein involved in a nonlysosomal ATP/ubiquitin-dependent proteolytic pathway. The protein is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. This protein highly expressed in prostate epithelium. Recombinant human PSMA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome subunit alpha type-1 isoform 2, HC2, Nu, PROS30
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
32 kDa (286aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
DeMartino GN., et al. (1991) Biochim Biophys Acta. 1079 (1) :29-38.; ; Silva Pereira I., et al. (1992) Gene. 120 (2) :235-42.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002777
Uniprot Accession Number
P25786
Species
Human
AA Sequence
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