Recombinant human PINK1 protein

PINK1 is a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this protein cause one form of autosomal recessive early-onset Parkinson disease. Recombinant human PINK protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serine/threonine-protein kinase PINK1 mitochondrial, Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, FLJ27236, PARK6

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.9 kDa (353aa)

Additionnal Information

PINK1, Serine/threonine-protein kinase PINK1 mitochondrial, Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, FLJ27236, PARK6, ATGP1240-10 µg, ATGP1240-20 µg, ATGP1240-50 µg, ATGP1240-100 µg, ATGP1240-250 µg, ATGP1240-500 µg, ATGP1240-1 mg, ATGP1240-10, ATGP1240-20, ATGP1240-50, ATGP1240-100, ATGP1240-250, ATGP1240-500, ATGP1240-1

References & Citations

Valente EM., et al. (2004) Ann Neurol. 56 (3) :336-41.; ; unoki M., et al. (2001) Oncogene. 20 (33) :4457-65.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 1M urea, 5% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

AAH28215

Uniprot Accession Number

Q9BXM7

Species

Human

AA Sequence

MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL