Recombinant human LAT protein

LAT is an integral membrane protein which is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells. Recombinant human LAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Linker for activation of T-cells family member 1 isoform b, LAT1, pp36

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.4 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

LAT, linker for activation of T-cells family member 1 isoform b, LAT1, pp36, ATGP1238-10 µg, ATGP1238-20 µg, ATGP1238-50 µg, ATGP1238-100 µg, ATGP1238-250 µg, ATGP1238-500 µg, ATGP1238-1 mg, ATGP1238-10, ATGP1238-20, ATGP1238-50, ATGP1238-100, ATGP1238-250, ATGP1238-500, ATGP1238-1

References & Citations

Paz PE., et al. (2001) Biochem J. 356 (2) :461-71.; ; Zhang W., et al. (1998) Cell. 92 (1) :83-92.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001014987

Uniprot Accession Number

O43561

Species

Human

AA Sequence

HCHRLPGSY DSTSSDSLYP RGIQFKRPHT VAPWPPAYPP VTSYPPLSQP DLLPIPRSPQ PLGGSHRTPS SRRDSDGANS VASYENEEPA CEDADEDEDD YHNPGYLVVL PDSTPATSTA APSAPALSTP GIRDSAFSME SIDDYVNVPE SGESAEASLD GSREYVNVSQ ELHPGAAKTE PAALSSQEAE EVEEEGAPDY ENLQELN