Recombinant human LAT protein
LAT is an integral membrane protein which is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells. Recombinant human LAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Linker for activation of T-cells family member 1 isoform b, LAT1, pp36
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
24.4 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Paz PE., et al. (2001) Biochem J. 356 (2) :461-71.; ; Zhang W., et al. (1998) Cell. 92 (1) :83-92.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001014987
Uniprot Accession Number
O43561
Species
Human
AA Sequence
Frequently Asked Questions
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