Recombinant human VHR/DUSP3 protein

DuSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DuSP3 is expressed in both breast and ovarian tissues. DuSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DuSP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Product Name Alternative

Dual specificity protein phosphatase 3, VHR, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, Vaccinia virus phosphatase VH1-related

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Molecular Weight

22.6 kDa (205aa) confirmed by MALDI-TOF

Additionnal Information

DUSP3, VHR, Dual specificity protein phosphatase 3, VHR, ATGP1237-10 µg, ATGP1237-20 µg, ATGP1237-50 µg, ATGP1237-100 µg, ATGP1237-250 µg, ATGP1237-500 µg, ATGP1237-1 mg, ATGP1237-10, ATGP1237-20, ATGP1237-50, ATGP1237-100, ATGP1237-250, ATGP1237-500, ATGP1237-1

References & Citations

Todd JL., et al. (1999) J Biol Chem. 274 (19) :13271-80.; ; Keyse SM., et al. (1995) Biochim Biophys Acta. 1265 (2-3) :152-60.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Scientific Category

Cancer

NCBI Accession Number

NP_004081

Uniprot Accession Number

P51452

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP