Recombinant human SNRPD3 protein
SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that comprises the spliceosome in eukaryotes. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. Recombinant human SNRPD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Small nuclear ribonucleoprotein Sm D3, Sm-D3, SMD3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
16 kDa (146aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lehmeier T. et al. (1995) Proc Natl Acad Sci. 91:12317-21.; ; Lehmeier T. et al. (1991) Nucleic Acids Res. 18: 6475-84.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.5M NaCl, 2mM DTT, 0.1mM PMSF
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004166
Uniprot Accession Number
P62318
Species
Human
AA Sequence
MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNKN QGSGAGRGKA AILKAQVAAR GRGRGMGRGN IFQKRR
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