Recombinant human SGK1 protein

SGK1, also known as serum-and glucocorticoid-regulated kinase, is a serine/threonine protein kinase and a member of the AGC subfamily, which includes protein kinases A, G, and C. This protein plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. It is activated in vitroby 3-phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivoin response to signals that activate phosphatidylinositol (PI) 3-kinase. Recombinant human SGK1, fused to His-tag at N-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serine/threonine-protein kinase Sgk1 isoform 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.5 kDa (393aa)

Additionnal Information

SGK1, Serine/threonine-protein kinase Sgk1 isoform 1, ATGP1232-10 µg, ATGP1232-20 µg, ATGP1232-50 µg, ATGP1232-100 µg, ATGP1232-250 µg, ATGP1232-500 µg, ATGP1232-1 mg, ATGP1232-10, ATGP1232-20, ATGP1232-50, ATGP1232-100, ATGP1232-250, ATGP1232-500, ATGP1232-1

References & Citations

Maiyar A.C. et al. (1996) J. Biol. Chem.. 271: 12414-12422.; ; Brunet A. et al. (2001) Mol. Cell. Biol. 21:952-65

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT,0.1mM PMSF

Scientific Category

Development

NCBI Accession Number

NP_005618

Uniprot Accession Number

O00141

Species

Human

AA Sequence

ISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL