Recombinant human PSMA2 protein

PSMA2, also known as proteasome subunit alpha type-2, is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMA2, belonged to the peptidase T1A family, is a 20S core alpha subunit of proteasome. This protein serves as docking domains for the regulatory particles and exterior gates blocking unregulated access to the interior cavity. Recombinant human PSMA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Proteasome subunit alpha type-2, HC3, Mu, PMSA2, PSC2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.0 kDa (254aa) confirmed by MALDI-TOF

Additionnal Information

PSMA2, Proteasome subunit alpha type-2, HC3, Mu, PMSA2, PSC2, ATGP1231-10 µg, ATGP1231-20 µg, ATGP1231-50 µg, ATGP1231-100 µg, ATGP1231-250 µg, ATGP1231-500 µg, ATGP1231-1 mg, ATGP1231-10, ATGP1231-20, ATGP1231-50, ATGP1231-100, ATGP1231-250, ATGP1231-500, ATGP1231-1

References & Citations

Cho S. et al. (2001) FEBS Lett. 498 (1) :62-6.; ; Dong J. et al. (2004) J Biol Chem. 279 (20) :21334-42.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002778.1

Uniprot Accession Number

P25787

Species

Human

AA Sequence

MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE GQMTEDNIEV GICNEAGFRR LTPTEVKDYL AAIA