Recombinant human NT5C2 protein

NT5C2, also known as NT5B or PNT5, has an essential role in the maintenance of purine/pyrimidine nucleotides. It contains a phosphotransferase active site that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, it regulates the level of inosine monophosphate (IMP) and guanosine monophosphate (GMP) pools within cells via hydrolysis. Recombinant human NT5C2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Cytosolic purine 5'-nucleotidase, cN-II, GMP. NT5B, PNT5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

67.1 kDa (581aa)

Additionnal Information

NT5C2, Cytosolic purine 5'-nucleotidase, cN-II, GMP. NT5B, PNT5, ATGP1229-10 µg, ATGP1229-20 µg, ATGP1229-50 µg, ATGP1229-100 µg, ATGP1229-250 µg, ATGP1229-500 µg, ATGP1229-1 mg, ATGP1229-10, ATGP1229-20, ATGP1229-50, ATGP1229-100, ATGP1229-250, ATGP1229-500, ATGP1229-1

References & Citations

Oka J. et al. (1994) Biochem. Biophys. Res. Commun. 205: 917-922.; ; Wallde K. et al. (2007) J Biol Chem. 282: 17828-17836.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_036361.1

Uniprot Accession Number

P49902

Species

Human

AA Sequence

MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E