Recombinant human NAPG protein

NAPG, also known as gamma-SNAP, is a cytoplasmic protein that binds to a membrane receptor complex composed of VAMP, SNAP25 and syntaxin. It mediates the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP25 and syntaxins for VAMP binding. Recombinant human NAPG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Gamma-soluble NSF attachment protein, GAMMA-SNAP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.3 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

NAPG, Gamma-soluble NSF attachment protein, GAMMA-SNAP, ATGP1228-10 µg, ATGP1228-20 µg, ATGP1228-50 µg, ATGP1228-100 µg, ATGP1228-250 µg, ATGP1228-500 µg, ATGP1228-1 mg, ATGP1228-10, ATGP1228-20, ATGP1228-50, ATGP1228-100, ATGP1228-250, ATGP1228-500, ATGP1228-1

References & Citations

Bennett M.K. et al. (1993) Cell 74: 863-873.; ; McMahon H.T. et al. (1995) J. Biol. Chem. 270: 2213-2217.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_003817.1

Uniprot Accession Number

Q99747

Species

Human

AA Sequence

MAAQKI NEGLEHLAKA EKYLKTGFLK WKPDYDSAAS EYGKAAVAFK NAKQFEQAKD ACLREAVAHE NNRALFHAAK AYEQAGMMLK EMQKLPEAVQ LIEKASMMYL ENGTPDTAAM ALERAGKLIE NVDPEKAVQL YQQTANVFEN EERLRQAVEL LGKASRLLVR GRRFDEAALS IQKEKNIYKE IENYPTCYKK TIAQVLVHLH RNDYVAAERC VRESYSIPGF NGSEDCAALE QLLEGYDQQD QDQVSDVCNS PLFKYMDNDY AKLGLSLVVP GGGIKKKSPA TPQAKPDGVT ATAADEEEDE YSGGLC