Recombinant human Keratin 14/KRT14 protein

KRT14, also known as keratin 14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs) . It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis. Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

53.8 kDa (492aa)

Additionnal Information

KRT14, Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI, ATGP1225-10 µg, ATGP1225-20 µg, ATGP1225-50 µg, ATGP1225-100 µg, ATGP1225-250 µg, ATGP1225-500 µg, ATGP1225-1 mg, ATGP1225-10, ATGP1225-20, ATGP1225-50, ATGP1225-100, ATGP1225-250, ATGP1225-500, ATGP1225-1

References & Citations

Romano RA. et al. (2007) J Invest Dermatol. 127:1175-1186.; ; Coulombe PA. et al. (1991) Cell. 66 (6) : 1301-11.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Cytoskeleton

NCBI Accession Number

AAH19097

Uniprot Accession Number

P02533

Species

Human

AA Sequence

MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN