Recombinant human GNAI1 protein

GNAI1, also known as guanine nucleotide-binding protein G (i) subunit alpha, functions to relay information from cell surface receptors to intracellular effectors. Also this protein functions to open atrial potassium channels. Recombinant human GNAI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Guanine nucleotide-binding protein G (i) subunit alpha, Gi

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

42.7 kDa (377aa) confirmed by MALDI-TOF

Additionnal Information

GNAI1, Guanine nucleotide-binding protein G (i) subunit alpha, Gi, ATGP1224-10 µg, ATGP1224-20 µg, ATGP1224-50 µg, ATGP1224-100 µg, ATGP1224-250 µg, ATGP1224-500 µg, ATGP1224-1 mg, ATGP1224-10, ATGP1224-20, ATGP1224-50, ATGP1224-100, ATGP1224-250, ATGP1224-500, ATGP1224-1

References & Citations

Jones D.T. et al. (1990) J Biol Chem. 265: 2671-2676.; ; Simon M.I. et al. (1991) Science 252: 802-808.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002060.4

Uniprot Accession Number

P63096

Species

Human

AA Sequence

MGCTLSA EDKAAVERSK MIDRNLREDG EKAAREVKLL LLGAGESGKS TIVKQMKIIH EAGYSEEECK QYKAVVYSNT IQSIIAIIRA MGRLKIDFGD SARADDARQL FVLAGAAEEG FMTAELAGVI KRLWKDSGVQ ACFNRSREYQ LNDSAAYYLN DLDRIAQPNY IPTQQDVLRT RVKTTGIVET HFTFKDLHFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTDTSIILF LNKKDLFEEK IKKSPLTICY PEYAGSNTYE EAAAYIQCQF EDLNKRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKDCGLF