Recombinant human Blk protein

BLK, also known as B lymphoid kinase, is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells. Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tyrosine-protein kinase Blk, MGC10442, MODY11

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 80% by SDS-PAGE

Molecular Weight

59.8 kDa (525aa)

Additionnal Information

Blk, Tyrosine-protein kinase Blk, MGC10442, MODY11, ATGP1223-10 µg, ATGP1223-20 µg, ATGP1223-50 µg, ATGP1223-100 µg, ATGP1223-250 µg, ATGP1223-500 µg, ATGP1223-1 mg, ATGP1223-10, ATGP1223-20, ATGP1223-50, ATGP1223-100, ATGP1223-250, ATGP1223-500, ATGP1223-1

References & Citations

Dymecki. et al. (1990) Science. 247:332-6.; ; Tatosyan, A.G. et al. (2000) Biochemistry. 65:49-58

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50% glycerol, 0.25M NaCl, 5mM DTT, 0.1mM PMSF, 0.1mM EDTA

Scientific Category

Apoptosis

NCBI Accession Number

NP_001706

Uniprot Accession Number

P51451

Species

Human

AA Sequence

MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP