Recombinant human Blk protein
BLK, also known as B lymphoid kinase, is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells. Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tyrosine-protein kinase Blk, MGC10442, MODY11
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 80% by SDS-PAGE
Molecular Weight
59.8 kDa (525aa)
Additionnal Information
References & Citations
Dymecki. et al. (1990) Science. 247:332-6.; ; Tatosyan, A.G. et al. (2000) Biochemistry. 65:49-58
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50% glycerol, 0.25M NaCl, 5mM DTT, 0.1mM PMSF, 0.1mM EDTA
Scientific Category
Apoptosis
NCBI Accession Number
NP_001706
Uniprot Accession Number
P51451
Species
Human
AA Sequence
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